BIOINFORMATICS
I need to use Galaxy to set up an experiment on some givenfiles, but I am unsure how to begin.
- I am distributing a Galaxy History called:CG19FinalProjectDNADatasets containing:
- Sacce1_AssemblyScaffolds.fasta <- Saccharomycescerevisiae Wild-Type Genome
- Sacce1.ExternalModels.gff3 <- Saccharomycescerevisiae Wild-Type Genome Annotations
- Scerevisiae_RAMutant_Genome.v02.fa <- Saccharomycescerevisiae Mutant Genome
- Sacce1_chrVI-VII-VIII.fasta <- Saccharomycescerevisiae Chromosomes VI/VII/VIII Wild-Type Genome
- All Datasets are from the Wild-TypeGenome
- Dataset01:
- SRR352492.sra_1.fastq
- SRR352492.sra_2.fastq
- Dataset02:
- SRR352384.sra_1.fastq
- SRR352384.sra_2.fastq
- Dataset03:
- SRR452441.csra_1.fastq
- SRR452441.csra_2.fastq
- The Datasets have already been Quality Controlled
- Saccharomyces cerevisiae Wild-Type ChromosomesVI/VII/VIII is being provided for you to test/optimize your mappingconditions
- Your task is:
- To use these NGS Datasets to identify any polymorphisms thatmight be present in theSaccharomyces cerevisiae MutantGenome
- You are welcome to validate your findings using other methods(e.g., alignments, Blast, etc), but your
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