Course Solutions Uncategorized (Answered) : Please Help Determine Cfu Ml Colonies Solve Average Recombinant Cells Percent Recombinatio Q28247410

(Answered) : Please Help Determine Cfu Ml Colonies Solve Average Recombinant Cells Percent Recombinatio Q28247410

Please help, how can i determine the cfu/ml for the coloniesand how do i solve average # of recombinant cells & percentrecombination
A640 nm of Hfr Cells Number of Hfr (Cfu/ml) from standard curve Number of Hfr (Cfu/ml) in reaction 8. Xo (Cfu/ml from graph x 2[dilution factor Growth of MMD plus streptomycin minus leucine (+/-) Growth on LB plates (+1-) Organisnm F- Hfr Colonies per plate Cfu/ml F- and Hfr Mix 10 102 10 101 MMD is minimal medium supplemented with amino acids necessary for growth of F. The indicated amino acid leucine UTC has been left out of the medium. Average number of recombinant cells (Cfu/ml) Percent recombinationA640

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